// Total dF/F Normalization

stk = getTitle(); 
stkne = File.getNameWithoutExtension(stk)

run("Z Project...", "projection=[Average Intensity]");
mean = getTitle(); 

// Calculate difference
imageCalculator("Subtract create 32-bit stack", stk, mean);
diff = getTitle(); 

// Normalize
imageCalculator("Divide create 32-bit stack", diff, mean);

rename(stk+"_dff");
setMinAndMax(-1, 1);

close(diff); 
close(mean);