// OHSU Slice Imaging Overarching process. // ================================================== // Written by Wilson Adams | Siciliano Lab | Feb 2022 // ================================================== // For each animal, we have a series of experiments to explore results from. Many of them are repeated stimulations on the same field of view. // To get longitudinal information about cells across multiple stim conditions, we need a way to track cells between imaging experiments to compare their responses as a function of stim time and amplitude. // For a series of ten experiments, we want to isolate and align the images of all 10 experiments. // Initially, we will need to segment our base stack to register all of our images against. To combat issues with photobleaching and whatnot, I am going to use the first imaging experiment in each group as the base image stack to register against (brightest baseline signal, least photobleaching). // Processing base stack // 1) Import base stack (virtual) // 2) Obtain A