// Difference Image Macro 

// Much like the Stack Normalization Tool, this macro will ratio two images in a stack of choice. 
// 	I tend to use this for Calcein images. 

stack = getTitle(); 

waitForUser("Scroll to Baseline Image Slice, then click 'OK'");
slice1 = getSliceNumber();



waitForUser("Scroll to Difference Image Slice, then click 'OK'");
slice2 = getSliceNumber();

selectWindow(stack);
run("Duplicate...", "duplicate range=&slice1-&slice1");
rename("Baseline");
base = getTitle();

selectWindow(stack);
run("Duplicate...", "duplicate range=&slice2-&slice2");
rename("difference")
diff = getTitle();

imageCalculator("Divide create 32-bit", diff, base);
final = getTitle()
rename(stack+"_differenceIMG")
close(base);
close(diff);