// Worm Data Processing

// Written by Wilson Adams, Vanderbilt Biophotonics Center, 24 July 2019

stk = getTitle()
makeRectangle(137, 49, 906, 931);

waitForUser('Selefct general field of interest')

roiManager("Add");
run("Enhance Contrast", "saturated=0.35");
run("Duplicate...", "duplicate");
roiManager("Select", 0);
//run("Crop");
run("Subtract Background...", "rolling=30 sliding stack");
run("Enhance Contrast", "saturated=0.35");