// Astro Virtual Crop 
//================================================================
run("Marker-controlled Watershed", "input=avgip_astropdl_001_316-2-Gradient marker=markers mask=avgip_astropdl_001_316-2 binary calculate use");
//================================================================
	    // Select Central Stim point on Any Img
	    setTool("point");
	    waitForUser("Select the central point of stimulation in the image");
	    roiManager("Add");
	    selectWindow("orig");
	    getSelectionCoordinates(xp, yp);
	    Array.print(Array.concat(xp, yp));

	    // Draw Rectangle centered on coordinate
	    makeRectangle(xp[0]-512, yp[0]-512, 1024, 1024);
		roiManager("delete")
		roiManager("add")
		
// Crop stack
run("Virtual Stack...", "output= &crp format=TIFF text1=[roiManager(\"Select\", 0);run(\"Crop\");]");