// F-SMAPTranslate.js script function by Christophe Leterrier // Translate single-channel txt localization files from .csv SMAP to .csv ThunderSTORM localization files importClass(Packages.java.io.File); importClass(Packages.java.io.FileReader); importClass(Packages.java.io.FileWriter); importClass(Packages.java.io.BufferedReader); importClass(Packages.java.io.BufferedWriter); importClass(Packages.ij.IJ); importClass(Packages.java.lang.Double); function SMAPTranslate(inPath, outDir) { var inSep = ","; // separator for the input file var sep = ","; // separator for the output file var sigpara = 0.4; // factor for uncertainty var bgdefault = 25 // background standard deviation in photons (fixed because not in Ries csv file) // Fields of the input header var inHeaderList = ["xnm","ynm","znm","layer","PSFxnm","PSFypix","bg","frame","locprecnm","locprecznm","logLikelihood","numberInGroup","phot","LLrel","PSFxpix","PSFynm","channel","colorfield","filenumber","groupindex","iterations","photerr","xnmerr","xpix","xpixerr","ynmerr","ypix","ypixerr"]; var outHeader2DList = ["\"frame\"","\"x [nm]\"","\"y [nm]\"","\"sigma [nm]\"","\"intensity [photon]\"","\"offset [photon]\"","\"bkgstd [photon]\"","\"uncertainty_xy [nm]\"", "\"chi2\"", "\"detections\""]; var outHeader3DList = ["\"frame\"","\"x [nm]\"","\"y [nm]\"","\"z [nm]\"","\"sigma1 [nm]\"","\"sigma2 [nm]\"","\"intensity [photon]\"","\"offset [photon]\"","\"bkgstd [photon]\"","\"uncertainty_xy [nm]\"","\"uncertainty_z [nm]\"", "\"chi2\"", "\"detections\""]; // Define input files, folder, open it etc. var inFile = new File(inPath); var inName = inFile.getName(); var inNameExt = getExt("" + inName); var br = new BufferedReader(new FileReader(inFile)); IJ.log(" inName: " + inName); // Get the header line and find index of Z, intensity, background and x coordinate columns var inHeader = br.readLine(); //IJ.log("inHeader: " + inHeader); var inHeaderArray = inHeader.split(inSep); // get the indexes of the columns needed in input file from its header var fIndex = arrayFind(inHeaderArray, inHeaderList[7]); var xIndex = arrayFind(inHeaderArray, inHeaderList[0]); var yIndex = arrayFind(inHeaderArray, inHeaderList[1]); var sdxIndex = arrayFind(inHeaderArray, inHeaderList[4]); var sdyIndex = arrayFind(inHeaderArray, inHeaderList[15]); var unxIndex = arrayFind(inHeaderArray, inHeaderList[22]); var unyIndex = arrayFind(inHeaderArray, inHeaderList[25]); var unxyIndex = arrayFind(inHeaderArray, inHeaderList[8]); var intIndex = arrayFind(inHeaderArray, inHeaderList[12]); var bgIndex = arrayFind(inHeaderArray, inHeaderList[6]); var chi2Index = arrayFind(inHeaderArray, inHeaderList[10]); var detecIndex = arrayFind(inHeaderArray, inHeaderList[11]); var zIndex = arrayFind(inHeaderArray, inHeaderList[2]); var unzIndex = arrayFind(inHeaderArray, inHeaderList[9]); // Define output extension and header depending on 2D or 3D if (zIndex == -1) { var is3D = false; var outSuffix = "_TS2D"; var outHeaderList = outHeader2DList; } else { var is3D = true; var outSuffix = "_TS3D"; var outHeaderList = outHeader3DList; } // Generate output name and path, open file writer var outName = inName.replace("." + inNameExt[1], outSuffix + ".csv"); if (outName == inName) outName = inNameExt[0] + outSuffix + "." + inNameExt[1]; // in the case we are processing CSV files var outPath = outDir + outName; var outFile = new File(outPath); if (!outFile.exists()) { outFile.createNewFile(); } var bw = new BufferedWriter(new FileWriter(outFile)); IJ.log(" outName: " + outName); // Write the header var outHeader = makeLineFromArray(outHeaderList, sep); bw.write(outHeader); bw.newLine(); // Write the output file line by line while ((inLine = br.readLine()) != null) { // Processing of individual values from input file lines, split by inSep var inCells = inLine.split(inSep); var fOut = inCells[fIndex]; var xOut = parseFloat(inCells[xIndex]).toFixed(1); var yOut = parseFloat(inCells[yIndex]).toFixed(1); var sigma1Out = parseFloat(inCells[sdxIndex]); var sigma2Out = parseFloat(inCells[sdyIndex]); if (is3D == false) var sigmaOut = (Math.sqrt((sigma1Out * sigma1Out) + (sigma2Out * sigma2Out))).toFixed(1); // var unxOut = parseFloat(inCells[unxIndex]); // var unyOut = parseFloat(inCells[unyIndex]); var unxyOut = (parseFloat(inCells[unxyIndex]) * sigpara).toFixed(1); var intOut = parseFloat(inCells[intIndex]).toFixed(0); var offOut = parseFloat(inCells[bgIndex]).toFixed(0); var bgstdOut = bgdefault; var chi2Out = parseFloat(inCells[chi2Index]).toFixed(2); var detecOut = inCells[detecIndex]; if (is3D == true) { var zOut = parseFloat(inCells[zIndex]).toFixed(1); var unzOut = (parseFloat(inCells[unzIndex]) * sigpara).toFixed(1); } // Assemble output line // 1 at the end is for the detection number (linked localization accross several frames) that are not reported by DOM if (is3D == false) var outLineArray = [fOut, xOut, yOut, sigmaOut, intOut, offOut, bgstdOut, unxyOut, chi2Out, detecOut]; else var outLineArray = [fOut, xOut, yOut, zOut, sigma1Out, sigma2Out, intOut, offOut, bgstdOut, unxyOut, unzOut, chi2Out, detecOut]; var outLine = makeLineFromArray(outLineArray, sep); // Write new line bw.write(outLine); bw.newLine(); } br.close(); bw.close(); } function getExt(filestring){ var namearray = filestring.split("."); var shortname = ""; for (var f = 0; f < namearray.length - 1; f++) { shortname = shortname + namearray[f]; } return [shortname, namearray[namearray.length - 1]]; } function arrayFind(a, s){ for (var i = 0; i < a.length; i++) { testS = a[i]; if (testS.indexOf(s)>-1 && testS.indexOf(s)<3) return i; } return -1; } function makeLineFromArray(ar, se) { ol = "" + ar[0]; for (t = 1; t < ar.length; t++) { ol = ol + se + ar[t]; } return ol; }