; ---- Eliminate the tiny, unwanted connections with a cutoff in the ; (cell_area, worst_bounding_path_score) plane ---- ; if outside cutoff, remove the worst_bounding_path ; ---- Segmentation & Unique Path Labeling ---- (ns Path_Finder.tinycell [:use Path_Finder.drawing] [:use [clojure.pprint :only [print-table pprint]]] [:import BinaryLabel_]) ; (use '[Path_Finder.main_ :only [params]]) ; (use '[Path_Finder.pathfinder :only [path_img paths scores]]) ; (def path_img (make-16bit-img "Paths" "blue orange icb" (.getWidth mem) (.getHeight mem))) ; (def paths ) ; (def good_path_ids (range 0 )) (defn remove-tiny-cells [path_img good_path_ids paths scores f_minarea] ; (def path_img_b (make-16bit-img "Paths" "blue orange icb" (.getWidth path_img) (.getHeight path_img))) ; (def path_proc_b (.convertToByte (.getProcessor path_img) true)) ; (.threshold path_proc_b 0) ; (.setProcessor path_img_b path_proc_b) ; (exec [ImagePlus Name white-particles? 4-connected?]) ; (let [result (.exec (BinaryLabel_.) path_img_b "Labelled" false true)] ; (def result_img (aget result 1))) (def result_img (get-cell-img path_img)) ; convert default 32-bit imgs. 16-bit is still big enough to uniquely label paths (def result_proc (.. result_img getProcessor (convertToShort false))) (.setProcessor result_img result_proc) (.show result_img) ; add paths to img mapped by unique-id (let [paths (map #(paths %) good_path_ids) big_ids (map #(+ 1000 %) good_path_ids)] ; add 1000 so we don't confuse paths with cells! (redraw-paths (map list paths big_ids) result_proc)) ; (.close path_img_b) (ij.IJ/log "Creating Mapping…") ; ---- Create Cell → [Paths] mapping ---- (def mapping (atom {})) (doseq [i (range 1 (dec (.getWidth path_img))) j (range 1 (dec (.getHeight path_img))) :let [mykey (.get result_proc i j)] :when (< 1 mykey 1000)] ; ignore background labelled 1 (let [ ; values of 4-connected neibs nvals (map #(.get result_proc (+ i %1) (+ j %2)) [1 -1 0 0] [0 0 1 -1]) ; keep the ones that are path-ids gdns (filter #(> % 1000) nvals) ; get set of values mapped-to by key ; if it's new, then return the empty set ; then add all the new path values to the set s (into (@mapping mykey #{}) gdns)] ; update mapping with the new set! (swap! mapping #(assoc % mykey s)))) (ij.IJ/log "Done with Mapping") ; Now use `mapping` to eliminate the small cells (def histo (.getHistogram result_proc)) (def cell_ids (range 1 (inc (count @mapping)))) (def cell_area (map #(aget histo %) cell_ids)) ; now all cells need to go through the testing... (def small_cells (filter #(> 64 (first %)) (map list cell_area cell_ids))) (def path_sets (map (fn [[ca cid]] [(@mapping cid) ca cid]) small_cells)) (let [f1 (fn [elem] (- elem 1000)) ; convert back to normal path_id f2 (fn [elem] [elem (scores elem)]) ; pair each bounding path with it's score f3 (fn [set] (map (comp f2 f1) set)) ; do above for each path bounding cell f4 (fn [lst] (sort-by second lst))] ; sort resulting paths by score ; get the highest scoring path_id for each cell in a [[path_id score] cell_area] tuple (def paths_to_remove (map (fn [[ps ca]] [((comp last f4 f3) ps) ca]) path_sets))) ; (def f_minarea (Path_Finder.main_/params :minarea)) (def paths_to_remove_2 (filter (fn [[[pid scr] ca]] (< (f_minarea ca) scr)) (filter first paths_to_remove))) (println "The paths to remove are: ") ; (pprint paths_to_remove_2) (.close result_img) ; this gives a list of [path_id score] to remove (set (map (comp first first) paths_to_remove_2)))