#Jeff Hardin, Dept. of Integrative Biology #Univ. of Wisconsin-Madison #jdhardin@wisc.edu #Current version July 26, 2025 #This script imports data from one or more CSV files in the form x[i],y[i] as paired columns #Using JFreeChart allows export as an editable SVG file. #User asked to choose legend placement and data structure from org.jfree.chart import ChartFactory, ChartPanel, JFreeChart, LegendItem from org.jfree.chart.title import LegendTitle from org.jfree.chart.annotations import XYTitleAnnotation from org.jfree.chart.plot import PlotOrientation, XYPlot from org.jfree.data.xy import XYDataset, XYSeries, XYSeriesCollection from org.jfree.chart.plot import PlotOrientation, XYPlot from org.jfree.data.xy import XYDataset, XYSeries, XYSeriesCollection from org.jfree.data.category import DefaultCategoryDataset from org.jfree.chart.ui import RectangleEdge,RectangleInsets,RectangleAnchor from javax.swing import JFrame import java.awt.Color as Color from java.awt import Dimension, BasicStroke import java.awt.Frame as Frame import java.awt.Window as Window from java.io import File as File from java.lang import System as System from ij import WindowManager as WindowManager from ij.plugin.frame import RoiManager as RoiManager from ij.process import ImageStatistics as ImageStatistics from ij.measure import Measurements as Measurements from ij import IJ as IJ from ij.measure import CurveFitter as CurveFitter from ij.gui import Plot as Plot from ij.gui import PlotWindow as PlotWindow from ij.gui import ImageWindow as ImageWindow from ij.gui import GenericDialog from ij import ImagePlus as ImagePlus from ij.io import FileInfo as FileInfo from ij.measure import ResultsTable as ResultsTable from ij import WindowManager as WindowManager import math import os from os import path, mkdir import csv #below from #https://stackoverflow.com/questions/736043/checking-if-a-string-can-be-converted-to-float-in-python def isfloat(value): try: float(value) return True except ValueError: return False def doPlot(): lineseparator = "\n" cellseparator = "," #plotColor=[] #plotColor = ("black", "red", "blue", "#32a852", "#7132a8", "#9C2005", "#754F04", "magenta", "darkGray", "gray", "lightGray", "green", "#9C6905", "cyan", "orange", "pink", "yellow"); #Call below is a feature of Fiji/IJ2 #See https://imagej.net/scripting/parameters k = len(listOfPaths) ## Ask if a single X and multiple Y columns or paired columns gd = GenericDialog("Options") #dataStructure = ["Single X", "Paired XY"] #legendPosition = ["Jfree default", "Inside plot"] gd.addRadioButtonGroup("Data structure:", ["Single X", "Paired XY"], 1,2,"Paired XY") gd.addRadioButtonGroup("Legend position:", ["JFree default", "Inside plot"], 1,2,"JFree default") gd.showDialog() if gd.wasOKed(): pairedChoice = gd.getNextRadioButton() legendPosition = gd.getNextRadioButton() else: return None for i in range(0, k): #read in FRAP curve data from CSV file #copies the whole file to an array of lines #have to type cast pathnames to Python str text_file = open(str(listOfPaths[i]), "r") #Use a Java method instead (need JVM 7+) file = File(str(listOfPaths[i])) #get file name using getName() filename = file.getName() #read whole file to a string data = text_file.read() #close file text_file.close() #separate into lines of text lines=data.split(lineseparator) numRows = len(lines) # recreates the column headers labels=lines[0].split(cellseparator) #get total columns numCol = len(labels) #Below is the equivalent calls using ImageJ's PLot functions #set up plot using ImageJ plot function #myPlot=Plot(filename, "X", "Y") #myPlot.setLineWidth(2) #IJ.open(str(listOfPaths[i])) #Use JFreeChart calls for plot #create series dataset = XYSeriesCollection() #Following was ImageJ plot legend call #legendString = labels[0] + " v. " + labels[1] if pairedChoice == "Paired XY": # If paired columns # set up plot using JFree plot function for m in range(0, numCol/2): x1=[] y1=[] series1 = XYSeries(labels[2*m] + " v. " + labels[2*m+1]) xMin = 0 xMax = 0 #Iterate over each row in the csv using reader object #assume header row; skip this, since we already did this above for j in range(1,numRows): row=lines[j].split(cellseparator) #print("Row:") #print(row) #print(len(row)) #added this because some CSV files have a trailing carriage return... if (len(row) > 1): #need to add code to check for empty cells, since ImageJ #produces CSV files in which there can be unequal numbers of rows with empty cells #Jython interpreter crashes when trying to convert to float if cell is blank #ImageJ plot functions are savvy about blank cells, but this code isn't! if ((isfloat(row[2*m])) and (isfloat(row[2*m+1]))): x1.append(float(row[2*m])) y1.append(float(row[2*m+1])) if (float(row[2*m]) < xMin): xMin = float(row[2*m]) if (float(row[2*m]) > xMax): xMax = float(row[2*m]) #Now add arrays to series and series to dataset for n in range(0, len(x1)-1): series1.add(x1[n],y1[n]) #print("Series:") #print(series1) dataset.addSeries(series1) #myPlot.setColor(plotColor[m]) #myPlot.add("Line",x1,y1) #if (m > 0): #legendString = legendString + "\t" + labels[2*(m)] + " v. " + labels[2*m+1] #now create the chart chart = ChartFactory.createXYLineChart(filename, "X", "Y", dataset) plot = chart.getPlot() r = plot.getRenderer() plot.setBackgroundPaint(Color.white) #set first series to black and let JFreeCHart set other colors r.setSeriesPaint(0, Color.BLACK) #Run through series and set each to 2 pt using this call in a loop for m in range(0, numCol/2): r.setSeriesStroke(m,BasicStroke(float(2.0))) myDimension = Dimension(600,400) chart.title.setVisible(False) chartPanel = ChartPanel(chart) chartPanel.setPreferredSize(myDimension) chartRectangleInserts = RectangleInsets(0, 0, 0, 10) chart.setPadding(chartRectangleInserts) #Manually set max for X axis; default. #org.jfree.chart.axis.ValueAxis.autoAdjustRange() doesn't give #great results, as it pads the X axis to much. domainAxis = plot.getDomainAxis() domainAxis.setRange(xMin,xMax) legend = chart.getLegend() #next lines draw legend using defaults if (legendPosition == "JFree default"): legend.setPosition(RectangleEdge.RIGHT) legend.setBorder(1,1,1,1) else: #Draw legend inside the plot, since deafult position is outside the plot #from JFreeChart samp[e code legend.setVisible(False) lt = LegendTitle(plot) lt.setPosition(RectangleEdge.BOTTOM) lt.setBorder(1,1,1,1) lt.setBackgroundPaint(Color.WHITE) ta = XYTitleAnnotation(0.98, 0.02, lt,RectangleAnchor.BOTTOM_RIGHT) ta.setMaxWidth(0.6) plot.addAnnotation(ta) # Show the chart in an interactive window # where the right-click menu enables saving to PNG or SVG, and adjusting properties frame = JFrame(filename) frame.getContentPane().add(chartPanel) frame.pack() frame.setVisible(True) else: # If single X and multiple Y columns # set up plot using JFree plot function for m in range(1, numCol): x1=[] y1=[] series1 = XYSeries(labels[m]) #series1 = XYSeries(labels[0] + " v. " + labels[m]) xMin = 0 xMax = 0 #Iterate over each row in the csv using reader object #assume header row; skip this, since we already did this above for j in range(1,numRows): row=lines[j].split(cellseparator) #print("Row:") #print(row) #print(len(row)) #added this because some CSV files have a trailing carriage return... if (len(row) > 1): #need to add code to check for empty cells, since ImageJ #produces CSV files in which there can be unequal numbers of rows with empty cells #Jython interpreter crashes when trying to convert to float if cell is blank #ImageJ plot functions are savvy about blank cells, but this code isn't! if ((isfloat(row[0])) and (isfloat(row[m]))): x1.append(float(row[0])) y1.append(float(row[m])) if (float(row[0]) < xMin): xMin = float(row[0]) if (float(row[0]) > xMax): xMax = float(row[0]) #Now add arrays to series and series to dataset for n in range(0, len(x1)-1): series1.add(x1[n],y1[n]) #print("Series:") #print(series1) dataset.addSeries(series1) #myPlot.setColor(plotColor[m]) #myPlot.add("Line",x1,y1) #if (m > 0): #legendString = legendString + "\t" + labels[2*(m)] + " v. " + labels[2*m+1] #now create the chart chart = ChartFactory.createXYLineChart(filename, "X", "Y", dataset) plot = chart.getPlot() r = plot.getRenderer() plot.setBackgroundPaint(Color.white) #set first series to black and let JFreeCHart set other colors r.setSeriesPaint(0, Color.BLACK) #Run through series and set each to 2 pt using this call in a loop for m in range(0, numCol/2): r.setSeriesStroke(m,BasicStroke(float(2.0))) myDimension = Dimension(600,400) chart.title.setVisible(False) chartPanel = ChartPanel(chart) chartPanel.setPreferredSize(myDimension) chartRectangleInserts = RectangleInsets(0, 0, 0, 10) chart.setPadding(chartRectangleInserts) #Manually set max for X axis; default. #org.jfree.chart.axis.ValueAxis.autoAdjustRange() doesn't give #great results, as it pads the X axis to much. domainAxis = plot.getDomainAxis() domainAxis.setRange(xMin,xMax) legend = chart.getLegend() if (legendPosition == "JFree default"): legend.setPosition(RectangleEdge.RIGHT) legend.setBorder(1,1,1,1) else: #Draw legend inside the plot, since deafult position is outside the plot #from JFreeChart samp[e code legend.setVisible(False) lt = LegendTitle(plot) lt.setPosition(RectangleEdge.BOTTOM) lt.setBorder(1,1,1,1) lt.setBackgroundPaint(Color.WHITE) ta = XYTitleAnnotation(0.98, 0.02, lt,RectangleAnchor.BOTTOM_RIGHT) ta.setMaxWidth(0.6) plot.addAnnotation(ta) # Show the chart in an interactive window # where the right-click menu enables saving to PNG or SVG, and adjusting properties frame = JFrame(filename) frame.getContentPane().add(chartPanel) frame.pack() frame.setVisible(True) #main #@ File[] listOfPaths (label="select files", style="files") doPlot()