// Batch NSTORM into ThunderSTORM script by Christophe Leterrier // Split and translate txt localization files from Nikon NSTORM to .csv ThunderSTORM localization files // Calls F-NSseqSplit.js, F-NStxtSplit.js, and F-NStxtTranslate.js // See https://github.com/cleterrier/ChriSTORM/blob/master/Readme.md importClass(Packages.java.io.File); importClass(Packages.ij.io.DirectoryChooser); importClass(Packages.ij.IJ); importClass(Packages.ij.gui.GenericDialog); // get arguments if called from macro: (seq, xydrift, warp, zdrift, zfactor, ppc, xcorr) if (typeof(getArgument) == "function") { called = true; args = getArgument(); argarray = args.split(","); } else called = false; // Output format var outFormat = "TS"; var extFormat = "txt"; // Path to ChriSTORM folder var plugDir = IJ.getDirectory("imagej"); var csPath = plugDir + "scripts" + File.separator + "NeuroCyto" + File.separator + "ChriSTORM" + File.separator; var routinePath = csPath + "Routines" + File.separator; // Default options var seq_def = false; var xydrift_def = true; var warp_def = true; var zdrift_def = true; var zfactor_def = 2; var xcorr_def = 1.01615; // Get input directory (dialog or argument) if (called == false) { var dc = new DirectoryChooser("Choose a folder"); var inDir = dc.getDirectory() + File.separator; } else { inDir = argarray[0]; } var inDirFile = new File(inDir); var parDir = inDirFile.getParent() + File.separator; // Options (dialog or arguments) var gd = new GenericDialog("Translator Options"); if (called == false) { gd.addCheckbox("Sequential acquisition channels", seq_def); gd.addCheckbox("Use drift-corrected XY coordinates", xydrift_def); gd.addCheckbox("Use warp-corrected coordinates", warp_def); gd.addCheckbox("Use drift-corrected Z coordinates", zdrift_def); gd.addNumericField("Z uncertainty factor", zfactor_def, 1, 3, "* XY uncertainty"); gd.addNumericField("Astigmatic compression correction (1 for none)", xcorr_def, 5, 7, "" ); gd.addMessage("(3D only, 1.01615 for NSTORM#1, 0.955 for NSTORM#2)"); gd.showDialog(); var seq = gd.getNextBoolean(); var xydrift = gd.getNextBoolean(); var warp = gd.getNextBoolean(); var zdrift = gd.getNextBoolean(); var zfactor = gd.getNextNumber(); var xcorr = gd.getNextNumber(); } else { seq = argarray[1]; xydrift = argarray[2]; warp = argarray[3]; zdrift = argarray[4]; zfactor = argarray[5]; xcorr = argarray[6]; } if (gd.wasOKed() || called == true) { // Start var startTime = new Date().getTime(); IJ.log("\n\n*** Batch N-STORM into ThunderSTORM started ***"); IJ.log("Input folder: " + inDir); // Batch Split // Get the split function path and load if (seq == false) { var splitterJS = "F-NStxtSplit.js"; var splitterPath = routinePath + splitterJS; IJ.log("\nSplitter path:" + routinePath + splitterJS); load(splitterPath); } else { var splitter1JS = "F-NSseqSplit.js"; var splitterJS = "F-NStxtSplit.js"; var splitter1Path = routinePath + splitter1JS; var splitterPath = routinePath + splitterJS; IJ.log("\nSequence Splitter path:" + routinePath + splitter1JS); IJ.log("\Channel Splitter path:" + routinePath + splitterJS); load(splitter1Path); load(splitterPath); } // Define input folder, define and create output folder var inDirSplit = inDir; IJ.log("Split input folder: " + inDirSplit); if (seq == false) { var outDirSplit = parDir + "Locs Split" + File.separator; IJ.log("Split output folder: " + outDirSplit); var outDirSplitFile = new File(outDirSplit); if (!outDirSplitFile.exists()) { outDirSplitFile.mkdir(); } } else { var outDirSplit1 = parDir + "Locs Seq Split" + File.separator; var outDirSplit = parDir + "Locs Split" + File.separator; IJ.log("Split Seq output folder: " + outDirSplit1); IJ.log("Split Chan output folder: " + outDirSplit); var outDirSplit1File = new File(outDirSplit1); if (!outDirSplit1File.exists()) { outDirSplit1File.mkdir(); } var outDirSplitFile = new File(outDirSplit); if (!outDirSplitFile.exists()) { outDirSplitFile.mkdir(); } } // Get the file list from the input folder, batch process them using the split function if (seq == true) { IJ.log("\nSequential Splitting..."); var fileQueueS1 = getExtFiles(inDirSplit, extFormat); for (var f = 0; f < fileQueueS1.length; f++) { var inPath1 = fileQueueS1[f]; NSseqSplit(inPath1, outDirSplit1); } inDirSplit = outDirSplit1; } IJ.log("\nChannel Splitting..."); var fileQueueS = getExtFiles(inDirSplit, extFormat); for (var f = 0; f < fileQueueS.length; f++) { var inPath = fileQueueS[f]; NStxtSplit(inPath, outDirSplit); } // Batch Translate // Get the translate function path and load var translateJS = "F-NStxtTranslate.js"; var translatePath = routinePath + translateJS; IJ.log("\nTranslator path:" + routinePath + translateJS); load(translatePath); // Define input folder, define and create output folder // input folder for translation is the output folder from previous step (split) var inDirTranslate = outDirSplit; var outDirTranslate = parDir + "Locs " + outFormat + File.separator; IJ.log("Translate input folder: " + inDirTranslate); IJ.log("Translate output folder: " + outDirTranslate); var outDirTFile = new File(outDirTranslate); if (!outDirTFile.exists()) { outDirTFile.mkdir(); } // Get the file list from the input folder, batch process them using the translate function var fileQueueT = getExtFiles(inDirTranslate, "txt"); for (var f = 0; f < fileQueueT.length; f++) { inPath = fileQueueT[f]; NStxtTranslate(inPath, outDirTranslate, outFormat, xydrift, warp, zdrift, zfactor, xcorr); } // End var stopTime = new Date().getTime(); var Time = stopTime - startTime; IJ.log("\n*** Batch N-STORM into ThunderSTORM ended after " + Time / 1000 + " s ***\n\n\n"); if (called == true) out = outDirTranslate; } function getExtFiles(dirst, inext) { var dir = new File(dirst); var allfiles = dir.listFiles(); var extFiles = new Array(); for (var i = 0 ; i < allfiles.length; i++) { var name = allfiles[i].getName(); var ext = name.substring(name.lastIndexOf(".")+1); if (ext == inext) { extFiles.push(allfiles[i]); } } return extFiles; }