// F-TranslateDOM_TS.js script function by Christophe Leterrier // Translate localizations between DOM results table and TunderSTORM csv importClass(Packages.java.io.File); importClass(Packages.java.io.FileReader); importClass(Packages.java.io.FileWriter); importClass(Packages.java.io.BufferedReader); importClass(Packages.java.io.BufferedWriter); importClass(Packages.ij.IJ); importClass(Packages.java.lang.Double); // Parameters // inPath: input file path // outDir: output directory path // ppc: photons per count to trasnlate camera ADU to photons (for intensity) function TranslateDOMTS(inPath, outDir, ppc){ // Separators var inSep = "\t"; // input separator var sep = ","; // output separator // Fields of the input header var inHeaderList = ["X_(px)","Y_(px)","Frame_Number","X_(nm)","X_loc_error(nm)","Y_(nm)","Y_loc_error(nm)","Z_(nm)","Z_loc_error(nm)","Amplitude_fit","Amp_error","BGfit","BGfit_error","SD_X_(nm)","SD_X_error(nm)","SD_Y_(nm)","SD_Y_error(nm)","False positive","IntegratedInt","SNR","R2_fit","Iterations_fit"]; var outHeader2DList = ["\"frame\"","\"x [nm]\"","\"y [nm]\"","\"sigma [nm]\"","\"intensity [photon]\"","\"offset [photon]\"","\"bkgstd [photon]\"","\"uncertainty_xy [nm]\"", "\"chi2\"", "\"detections\""]; var outHeader3DList = ["\"frame\"","\"x [nm]\"","\"y [nm]\"","\"z [nm]\"","\"sigma1 [nm]\"","\"sigma2 [nm]\"","\"intensity [photon]\"","\"offset [photon]\"","\"bkgstd [photon]\"","\"uncertainty_xy [nm]\"","\"uncertainty_z [nm]\"", "\"chi2\"", "\"detections\""]; // Correspondance /* 2D case: Frame Number = frame [2] X_(nm) = x [nm] [3] Y_(nm) = y [nm] [4] average(SD_X_(nm), SD_Y_(nm))= sigma [nm] sqrt([14]^2 +[15]^2) average(X_loc_error(nm), Y_loc_error(nm)) = uncertainty_xy[nm] sqrt([4]^2 +[6]^2) IntegratedInt * PpC = intensity [photon] [18]*ppc BGfit * ppc = offset [photon] [11]*ppc BGfit_error * ppc = bkgstd [photon]? [12]*ppc R2_fit = chi2 [20] 3D case: Z_(nm) = z [nm][7] Z_loc_error(nm) = uncertainty_z[nm] [8] SD_X_(nm) = sigma1 [nm] [14] SD_Y_(nm) = sigma2 [nm] [15] */ // Define input files, folder, open it etc. var inFile = new File(inPath); var inName = inFile.getName(); var inNameExt = getExt("" + inName); var br = new BufferedReader(new FileReader(inFile)); IJ.log(" inName: " + inName); // Get the header line and find index of Z, intensity, background and x coordinate columns var inHeader = br.readLine(); //IJ.log("inHeader: " + inHeader); var inHeaderArray = inHeader.split(inSep); // get the indexes of the columns needed in input file from its header var fIndex = arrayFind(inHeaderArray, inHeaderList[2]); var xIndex = arrayFind(inHeaderArray, inHeaderList[3]); var yIndex = arrayFind(inHeaderArray, inHeaderList[5]); var sdxIndex = arrayFind(inHeaderArray, inHeaderList[14]); var sdyIndex = arrayFind(inHeaderArray, inHeaderList[15]); var unxIndex = arrayFind(inHeaderArray, inHeaderList[4]); var unyIndex = arrayFind(inHeaderArray, inHeaderList[6]); var intIndex = arrayFind(inHeaderArray, inHeaderList[18]); var bgIndex = arrayFind(inHeaderArray, inHeaderList[11]); var errbgIndex = arrayFind(inHeaderArray, inHeaderList[12]); var chi2Index = arrayFind(inHeaderArray, inHeaderList[20]); var zIndex = arrayFind(inHeaderArray, inHeaderList[7]); var unzIndex = arrayFind(inHeaderArray, inHeaderList[8]); // Define output extension and header depending on 2D or 3D if (zIndex == -1) { var is3D = false; var outSuffix = "_TS2D"; var outHeaderList = outHeader2DList; } else { var is3D = true; var outSuffix = "_TS3D"; var outHeaderList = outHeader3DList; } // Generate output name and path, open file writer var outName = inName.replace("." + inNameExt[1], outSuffix + ".csv"); if (outName == inName) outName = inNameExt[0] + outSuffix + "." + inNameExt[1]; // in the case we are processing CSV files var outPath = outDir + outName; var outFile = new File(outPath); if (!outFile.exists()) { outFile.createNewFile(); } var bw = new BufferedWriter(new FileWriter(outFile)); IJ.log(" outName: " + outName); // Write the header var outHeader = makeLineFromArray(outHeaderList, sep); bw.write(outHeader); bw.newLine(); // Write the output file line by line while ((inLine = br.readLine()) != null) { // Processing of individual values from input file lines, split by inSep var inCells = inLine.split(inSep); var fOut = inCells[fIndex]; var xOut = (parseFloat(inCells[xIndex])).toFixed(1); var yOut = (parseFloat(inCells[yIndex])).toFixed(1); var sigma1Out = parseFloat(inCells[sdxIndex]); var sigma2Out = parseFloat(inCells[sdyIndex]); if (is3D == false) var sigmaOut = (Math.sqrt((sigma1Out * sigma1Out) + (sigma2Out * sigma2Out))).toFixed(1); var unxOut = parseFloat(inCells[unxIndex]); var unyOut = parseFloat(inCells[unyIndex]); var unxyOut = (Math.sqrt((unxOut * unxOut) + (unyOut * unyOut))).toFixed(1); var intOut = (parseFloat(inCells[intIndex]) * ppc).toFixed(0); var offOut = (parseFloat(inCells[bgIndex]) * ppc).toFixed(0); var bgstdOut = (parseFloat(inCells[errbgIndex]) * ppc).toFixed(1); var chi2Out = (parseFloat(inCells[chi2Index])).toFixed(2); if (is3D == true) { var zOut = (parseFloat(inCells[zIndex])).toFixed(1); var unzOut = (parseFloat(inCells[unzIndex])).toFixed(1); } // Assemble output line // 1 at the end is for the detection number (linked localization accross several frames) that are not reported by DOM if (is3D == false) var outLineArray = [fOut, xOut, yOut, sigmaOut, intOut, offOut, bgstdOut, unxyOut, chi2Out, "1"]; else var outLineArray = [fOut, xOut, yOut, zOut, sigma1Out, sigma2Out, intOut, offOut, bgstdOut, unxyOut, unzOut, chi2Out, "1"]; var outLine = makeLineFromArray(outLineArray, sep); // Write new line bw.write(outLine); bw.newLine(); } br.close(); bw.close(); } function getExt(filestring){ var namearray = filestring.split("."); var shortname = ""; for (var f = 0; f < namearray.length - 1; f++) { shortname = shortname + namearray[f]; } return [shortname, namearray[namearray.length - 1]]; } function arrayFind(a, s){ for (var i = 0; i < a.length; i++) { testS = a[i]; if (testS.indexOf(s)>-1 && testS.indexOf(s)<3) return i; } return -1; } function makeLineFromArray(ar, se) { ol = "" + ar[0]; for (t = 1; t < ar.length; t++) { ol = ol + se + ar[t]; } return ol; }